The aim of the present was to develop and validate uv spectrophotometric method for analysis of antidiabetic drug Bougainvillea spectabilis (D-pinitol). Bougainvillea spectabilis is a most abundantly found plant having an insulin mimicking agent. A simple, accurate, sensitive, precise and economical spectroscopic method has been developed and validated for the determination of this drug. The solvent used was phosphate buffer (6.8) (for solubility purpose) to determine the D-pinitol. The wavelength corresponding to maximum absorbance of the D-pinitol was found at 205 nm. The method obeys Beer’s law in the concentration range of 50-450 μg/ml and exhibited good correlation coefficient (R2=0.9993) and the regression of the curve was found y=0.1379x-0.2467 with excellent mean recovery (98-100%). The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.69169 μg/ml and 2.03544 μg/ml respectively. The method was validated for several parameters like accuracy, precision as per ICH guidelines. The values of relative standard deviation and percentage recovery were found to be satisfactory; indicating that the proposed method is precise, accurate and economical hence can be used for the routine analysis.
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